The global folding of four-way helical junctions in RNA, including that in U1 snRNA

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چکیده

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The global folding of four-way helical junctions in RNA, including that in U1 snRNA

Helical junctions are important elements in the architecture of folded RNA molecules. The global geometry of fully base-paired four-way junctions between RNA helices has been analyzed by comparative gel electrophoresis. Junctions appear to fold by pairwise coaxial helical stacking in one of two possible stereochemically equivalent isomers based upon alternative selections of stacking partners. ...

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Single-molecule studies of DNA and RNA four-way junctions.

Branched helical junctions are common in nucleic acids. In DNA, the four-way junction (Holliday junction) is an essential intermediate in homologous recombination and is a highly dynamic structure, capable of stacking conformer transitions and branch migration. Our single-molecule fluorescence studies provide unique insight into the energy landscape of Holliday junctions by visualizing these pr...

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ژورنال

عنوان ژورنال: Cell

سال: 1995

ISSN: 0092-8674

DOI: 10.1016/0092-8674(95)90218-x